recombinant mouse cd38 Search Results


cd38 protein, mouse, recombinant  (TargetMol)
N/A
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mouse cd38 proteins  (R&D Systems)
92
R&D Systems mouse cd38 proteins
Fig. 4 | SASP increases <t>CD38</t> accumulation and decreases NAD+ levels in WAT in vivo. a,b, Twelve-month-old WT mice received daily s.c. injection of CM from CM-SEN or CM-NS mouse pre-adipocytes for 5 d, and tissues were harvested at day 5. a, CD38 activity (n = 7 mice per group) and NAD+ levels (CM-NS, n = 7 mice; CM-SEN, n = 6 mice) were measured in subcutaneous (subq) WAT. b, Relative Cd38 (n = 11 mice per group), Cd45 and F4/80 (CM-NS, n = 14 mice; CM-SEN, n = 12 mice) mRNA expression measured by RT–qPCR in subq WAT. Levels were relative to CM-NS. c–f, Twenty-six-month-old mice were treated with vehicle (ctrl) or 3TC for 2 weeks. c, Schematic of experiment. d, Relative mRNA levels for p16 and SASP components were measured by RT–qPCR in WAT (n = 8 mice per group for all conditions except Pai1 3TC (n = 7)). e, Relative mRNA levels for F4/80 (n = 8 mice per group), Cd38, Parp1 and Sirt1 (n = 5 mice per group) were measured by RT–qPCR in WAT. f, Relative NAD+ levels in WAT (n = 5 mice per group); levels were relative to vehicle-treated mice. g, Immunofluorescence staining of CD38 (red), lamin B1 (green) and IL-6 (yellow) in WAT of young (2-month-old) and old (28-month-old) C57BL/6 mice, representative of n = 4 young mice and n = 6 old mice. Insets show enlarged areas of old tissue, with and without IL-6 staining, to highlight that CD38 can be found near IL-6+lamin B1− cells. Data are the mean ± s.e.m., analysed by an unpaired two-sided t-test. Two statistical outliers were identified in the Cd45 mRNA expression of the CM-SEN-treated animals (with values >16) using the appropriate test in GraphPad Prism 6. These samples were excluded from all PCR analyses for this experiment. Data were also analysed by non-parametric Mann–Whitney test using the full dataset (including the identified statistical outliers); Cd45 P value = 0.002.
Mouse Cd38 Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+cd38/Recombinant+Mouse+CD38+Protein%2C+CF/pm33199925-417-3-22
Average 92 stars, based on 1 article reviews
mouse cd38 proteins - by Bioz Stars, 2026-10
92/100 stars
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recombinant mouse cd38 protein cf  (R&D Systems)
N/A
The Recombinant Mouse CD38 Protein from R D Systems is derived from NS0 The Recombinant Mouse CD38 Protein has been validated for the following applications Enzyme Activity
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cd38, his-tag (mouse), hip recombinant  (BPS Bioscience)
N/A
Mouse CD38, also known as cluster of differentiation 38 with C-terminal His-tag, GenBank Accession No. NM_007646, a.a. 45-303 expressed in a HEK293 cell expression system. MW = 31 kDa. This protein runs at a higher
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recombinant mouse cd38/adp-ribosyl cyclase 1/cyclic adp-ribose hydrolase 1  (Beijing Solarbio Science)
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recombinant mouse cd38 protein  (Elabscience Biotechnology)
N/A
CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, is a Signal-anchor for type II membrane protein. CD38 is able to transform NAD+ to ADP-D-ribose and nicotinamide. It also can transform NADP+ to nicotinate-adenine dinucleotide
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recombinant mouse cd38 protein  (Elabscience)
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mouse recombinant anti-human cd38 recombinant antibody  (Angio-Proteomie)
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recombinant anti-mouse cd38 single domain antibody  (Creative BioMart)
N/A
Anti-mouse CD38 Single Domain Antibody is a protein produced in E. coli.This product can be useful in applications such as Western blot; Immunohistochemistry; Flow Cytometry; Functional Studies and most other immunological methods.4°C. For long term
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Fig. 4 | SASP increases CD38 accumulation and decreases NAD+ levels in WAT in vivo. a,b, Twelve-month-old WT mice received daily s.c. injection of CM from CM-SEN or CM-NS mouse pre-adipocytes for 5 d, and tissues were harvested at day 5. a, CD38 activity (n = 7 mice per group) and NAD+ levels (CM-NS, n = 7 mice; CM-SEN, n = 6 mice) were measured in subcutaneous (subq) WAT. b, Relative Cd38 (n = 11 mice per group), Cd45 and F4/80 (CM-NS, n = 14 mice; CM-SEN, n = 12 mice) mRNA expression measured by RT–qPCR in subq WAT. Levels were relative to CM-NS. c–f, Twenty-six-month-old mice were treated with vehicle (ctrl) or 3TC for 2 weeks. c, Schematic of experiment. d, Relative mRNA levels for p16 and SASP components were measured by RT–qPCR in WAT (n = 8 mice per group for all conditions except Pai1 3TC (n = 7)). e, Relative mRNA levels for F4/80 (n = 8 mice per group), Cd38, Parp1 and Sirt1 (n = 5 mice per group) were measured by RT–qPCR in WAT. f, Relative NAD+ levels in WAT (n = 5 mice per group); levels were relative to vehicle-treated mice. g, Immunofluorescence staining of CD38 (red), lamin B1 (green) and IL-6 (yellow) in WAT of young (2-month-old) and old (28-month-old) C57BL/6 mice, representative of n = 4 young mice and n = 6 old mice. Insets show enlarged areas of old tissue, with and without IL-6 staining, to highlight that CD38 can be found near IL-6+lamin B1− cells. Data are the mean ± s.e.m., analysed by an unpaired two-sided t-test. Two statistical outliers were identified in the Cd45 mRNA expression of the CM-SEN-treated animals (with values >16) using the appropriate test in GraphPad Prism 6. These samples were excluded from all PCR analyses for this experiment. Data were also analysed by non-parametric Mann–Whitney test using the full dataset (including the identified statistical outliers); Cd45 P value = 0.002.

Journal: Nature metabolism

Article Title: CD38 ecto-enzyme in immune cells is induced during aging and regulates NAD + and NMN levels.

doi: 10.1038/s42255-020-00298-z

Figure Lengend Snippet: Fig. 4 | SASP increases CD38 accumulation and decreases NAD+ levels in WAT in vivo. a,b, Twelve-month-old WT mice received daily s.c. injection of CM from CM-SEN or CM-NS mouse pre-adipocytes for 5 d, and tissues were harvested at day 5. a, CD38 activity (n = 7 mice per group) and NAD+ levels (CM-NS, n = 7 mice; CM-SEN, n = 6 mice) were measured in subcutaneous (subq) WAT. b, Relative Cd38 (n = 11 mice per group), Cd45 and F4/80 (CM-NS, n = 14 mice; CM-SEN, n = 12 mice) mRNA expression measured by RT–qPCR in subq WAT. Levels were relative to CM-NS. c–f, Twenty-six-month-old mice were treated with vehicle (ctrl) or 3TC for 2 weeks. c, Schematic of experiment. d, Relative mRNA levels for p16 and SASP components were measured by RT–qPCR in WAT (n = 8 mice per group for all conditions except Pai1 3TC (n = 7)). e, Relative mRNA levels for F4/80 (n = 8 mice per group), Cd38, Parp1 and Sirt1 (n = 5 mice per group) were measured by RT–qPCR in WAT. f, Relative NAD+ levels in WAT (n = 5 mice per group); levels were relative to vehicle-treated mice. g, Immunofluorescence staining of CD38 (red), lamin B1 (green) and IL-6 (yellow) in WAT of young (2-month-old) and old (28-month-old) C57BL/6 mice, representative of n = 4 young mice and n = 6 old mice. Insets show enlarged areas of old tissue, with and without IL-6 staining, to highlight that CD38 can be found near IL-6+lamin B1− cells. Data are the mean ± s.e.m., analysed by an unpaired two-sided t-test. Two statistical outliers were identified in the Cd45 mRNA expression of the CM-SEN-treated animals (with values >16) using the appropriate test in GraphPad Prism 6. These samples were excluded from all PCR analyses for this experiment. Data were also analysed by non-parametric Mann–Whitney test using the full dataset (including the identified statistical outliers); Cd45 P value = 0.002.

Article Snippet: Recombinant human and mouse CD38 proteins, NMNAT1, anti-mouse TNF-α- and IL6-neutralizing antibodies, and Luminex Premixed Multi-Analyte Magnetic Luminex Assay kit were from R&D Systems. αMEM, DMEM, DMEM:F12, FCS, glutamine, ITS, penicillin–streptomycin, and Lipofectamine 3000 were from Life Technologies.

Techniques: In Vivo, Injection, Activity Assay, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining, MANN-WHITNEY

Fig. 9 | Schematic of the role of cellular senescence and sterile inflammation in the regulation of CD38 and NMN degradation. In young mice, levels of CD38+ inflammatory cells and senescent cells in tissues are lower than in aged mice. During aging, there is an increase in senescent cells that, in part, through their SASP, promotes accumulation of CD38+ immune cells. The ecto-enzymatic activity of CD38 in immune cells degrades NMN extracellularly, preventing the NMN-induced NAD+ boosting in other cells in the tissue.

Journal: Nature metabolism

Article Title: CD38 ecto-enzyme in immune cells is induced during aging and regulates NAD + and NMN levels.

doi: 10.1038/s42255-020-00298-z

Figure Lengend Snippet: Fig. 9 | Schematic of the role of cellular senescence and sterile inflammation in the regulation of CD38 and NMN degradation. In young mice, levels of CD38+ inflammatory cells and senescent cells in tissues are lower than in aged mice. During aging, there is an increase in senescent cells that, in part, through their SASP, promotes accumulation of CD38+ immune cells. The ecto-enzymatic activity of CD38 in immune cells degrades NMN extracellularly, preventing the NMN-induced NAD+ boosting in other cells in the tissue.

Article Snippet: Recombinant human and mouse CD38 proteins, NMNAT1, anti-mouse TNF-α- and IL6-neutralizing antibodies, and Luminex Premixed Multi-Analyte Magnetic Luminex Assay kit were from R&D Systems. αMEM, DMEM, DMEM:F12, FCS, glutamine, ITS, penicillin–streptomycin, and Lipofectamine 3000 were from Life Technologies.

Techniques: Sterility, Activity Assay